Intro on Lep-EU

The 10K Lep Genomes COST action (EU) is a large consortium whose aims are generating, sharing, and utilizing reference genomes and resequencing data for all European Lepidoptera.

LepEU is a group of researchers focused upon sampling populations of Lepidoptera species across Europe, to look for patterns of local adaptation across geography and through seasons. Our aim is to generate individual level re-sequencing data for diverse populations that are of shared interest to LepEU and 10KLepGenomes members.

This workshop is the 2025 gathering of LepEU and the Population genomics analyses Working group (WG4), supported by the COST action.

This meeting is for people to meet in person, organise our efforts forthis and coming years (collection, sequencing, analyses) and to start processing specimens and extract DNA for sequencing.

Meeting aims

  • Build the LepEU community
  • Define scientific objectives
  • Strengthen collaborations 
  • Discuss & plan 2025 field priorities
  • Plan and coordinate data acquisition
  • Coordinate collections, archiving, databasing.
  • Coordinate DNA isolations from 2024 collections
Who should come? We encourage researchers from all career stages that are interested in LepEU to come. There are a wide range of questions that regional sampling of European Lepidoptera could address. Let's see how we could make our individual efforts go further if we can collaborate together. We are inspired to keep building the LepEU community using a grass roots effort framework.
 
The total number of attendees we can fit is 30.
 

Preliminary schedule

12/05 Arrival of participants and meetup in town in the late afternoon

13/05 10K LepGenomes WG4 and LepEU Discussion at the CNRS campus

14/05 10K LepGenomes WG4 and LepEU Discussion at the CNRS campus

15/05 DNA extractions at the facility on the University campus (just nearby)

16/05 Excursion to meet with the Lepidoptera fauna in the Mediterranean surroudings.

Specimens for DNA isolation

For those needing DNA extraction help, we propose to provide a solution to isolate DNA during that week. This is aimed primarily to help people who might find it difficult to do that in their own institution. If you are interested, you need to register your samples so we can plan extraction consumables. We will reach out individually. We will come back to you individually with more information.

https://docs.google.com/spreadsheets/d/1CwING8qWnFGN0rCNn8sW6SBu1mj1QN095s8XgZRmUQU/edit?usp=sharing

Please bring your specimens already prepared into fragments of one third to one half of a thorax (depending on the size of the animal), preserved in 96% ethanol in 2ml eppendorf tubes. We will be providing kits and reagents.

We can host the DNA plates in my lab until arrangements for sequencing are found (no obligation). If you want to bring vouchers please do, they can then travel to Sweden with Chris Wheat, to be deposited in the Lund collection as offered by Niklas Wahlberg.

Attendance and support

Arriving and leaving

So we know when to expect whom, here is a small googleform for you to share your times of arrival and departure, if you don’t mind. https://forms.gle/4jinzK5YesoPk3G86

The airport is connected by an hourly bus that takes you to the tram station Place de l’Europe. It is often easier to take a taxi or uber. The fare is 35-40 euros normally.

The train station Saint-Roch is right in the city centre. There is another station called Sud de France, near the motorway, which is less well connected (a bus takes you to the tram, or to the central station) (avoid if you can)

Trains and coaches connect to Marseilles Airport or Vitrolles station. In Montpellier the coach stations are not in the city centre, but at the Sabines station or Odysseum station.

 

Dinner on Monday

Tables are booked for dinner at Le Pré Vert, 10 rue Sainte Anne, 3400 Montpellier from 7PM.

Because some may be arriving earlier, we will team up from 5PM at the nearby Bistrot Ste Anne, 7 place du Petit Scel.

 

Getting to the Venue

The meeting will take place at the Centre for evolutionary and functional ecology (CEFE – CNRS) on the CNRS campus.

Entrance to the campus is situated at : 1919 route de Mende, 34293, Montpellier.

To come by public transport (takes ~30min), several options (e.g. from Comédie):

-Tram line 1 to Saint-Eloi, and then connect to bus 22 to the stop CNRS.

-Tram line 1 to Universités Sciences et Lettres then connect to Bus A/B (shuttle bus between Universités des Sciences et Lettres) to Lavalette, then a short walk up route de Mende

-Tram line 2 to Aiguelongue, then Bus 10 to Moulin de Gasconnet, then walk from there.

-It is possible to walk from Saint-Eloi (20 min)

You can find info on the www.tam-voyages.com website but it’s all in French.

 

On Campus

You will be on the list of people authorized to enter the campus. Security may want you to leave an ID and give a swipe card in exchange, but in fact you won’t be needing a swipe card. The meeting will take place at the CEFE in Grande Salle de Réunion (1st floor, Corridor C). We will signpost the way. Lunch will be taken at the campus cantine, where you can pay by card.

 

Registration

The participants selected for reimbursement were notified in April. Members of WG4 who accepted the official invitation will have to sign a daily attence form (Brussels rules)

Contact

If you have any questions or need to discuss your participation please contact Mathieu Joron (+33 06-2828-1188, mathieu.joron@cefe.cnrs.fr) or Chris Wheat (chris.wheat@zoologi.su.se)

See you soon in Montpellier !

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